Glycosaminoglycan-binding properties and kinetic characterization of human heparin cofactor II expressed in Escherichia coli

Irreversible inactivation of α-thrombin (T) by the serpin, heparin cofactor II (HCII), is accelerated by ternary complex formation with the glycosaminoglycans (GAGs) heparin and dermatan sulfate (DS). Low expression of human HCII in Escherichia coli was optimized by silent mutation of 27 rare codons...

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Bibliographic Details
Main Authors: Sarilla, Suryakala, Habib, Sally Y., Tollefsen, Douglas M., Friedman, David B., Arnett, Diana R., Verhamme, Ingrid M.
Format: Artigo
Language:Inglês
Published: 2010
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2952836/
https://ncbi.nlm.nih.gov/pubmed/20670608
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2010.07.024
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