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Glycosaminoglycan-binding properties and kinetic characterization of human heparin cofactor II expressed in Escherichia coli
Irreversible inactivation of α-thrombin (T) by the serpin, heparin cofactor II (HCII), is accelerated by ternary complex formation with the glycosaminoglycans (GAGs) heparin and dermatan sulfate (DS). Low expression of human HCII in Escherichia coli was optimized by silent mutation of 27 rare codons...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2952836/ https://ncbi.nlm.nih.gov/pubmed/20670608 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2010.07.024 |
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