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Regulation of nitric oxide synthesis by proinflammatory cytokines in human umbilical vein endothelial cells. Elevations in tetrahydrobiopterin levels enhance endothelial nitric oxide synthase specific activity.

We have examined cytokine regulation of nitric oxide synthase (NOS) in human umbilical vein endothelial cells (HUVEC). 24-h treatment with IFN-gamma (200 U/ml) plus TNF (200 U/ml) or IL-1 beta (5 U/ml) increased NOS activity in HUVEC lysates, measured as conversion of [14C]L-arginine to [14C]L-citru...

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Udgivet i:J Clin Invest
Principais autores: Rosenkranz-Weiss, P, Sessa, W C, Milstien, S, Kaufman, S, Watson, C A, Pober, J S
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Clinical Investigation 1994
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC294374/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7514193/
https://ncbi.nlm.nih.govhttps://doi.org/10.1172/JCI117221
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