Optimization and validation of a robust human T cell culture method for monitoring phenotypic and polyfunctional antigen-specific CD4 and CD8 T-cell responses

BACKGROUND: Monitoring cellular immune responses is one prerequisite for the rational development of cancer vaccines. METHODS: Here, we describe an extensive effort to optimize and quantitatively validate an in vitro T cell culture method by determining the phenotype and function of both CD4(+) and...

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Main Authors: Lin, Yun, Gallardo, Humilidad F., Ku, Geoffrey Y., Li, Hao, Manukian, Gregor, Rasalan, Teresa S., Xu, Yinyan, Terzulli, Stephanie L., Old, Lloyd J., Allison, James P., Houghton, Alan N., Wolchok, Jedd D., Yuan, Jianda
Formato: Artigo
Idioma:Inglês
Publicado: 2009
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2932850/
https://ncbi.nlm.nih.gov/pubmed/19903103
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3109/14653240903136987
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