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Optimization and validation of a robust human T cell culture method for monitoring phenotypic and polyfunctional antigen-specific CD4 and CD8 T-cell responses
BACKGROUND: Monitoring cellular immune responses is one prerequisite for the rational development of cancer vaccines. METHODS: Here, we describe an extensive effort to optimize and quantitatively validate an in vitro T cell culture method by determining the phenotype and function of both CD4(+) and...
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| Hlavní autoři: | , , , , , , , , , , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
2009
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2932850/ https://ncbi.nlm.nih.gov/pubmed/19903103 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3109/14653240903136987 |
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