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High-yield Heterologous Expression of Wild Type and Mutant Ca(2+) ATPase: Characterization of Ca(2+) Binding Sites by Charge Transfer

High-yield heterologous SERCA1 (Ca(2+) ATPase) expression was obtained in COS-1 cells infected with recombinant adenovirus vector (rAdSERCA). Higher transcription and expression were obtained in the presence of a His(6) tag at the amino terminus, as compared with a His(6) tag at the carboxyl SERCA t...

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Bibliografske podrobnosti
Main Authors: Liu, Yueyong, Pilankatta, Rajendra, Lewis, David, Inesi, Giuseppe, Tadini-Buoninsegni, Francesco, Bartolommei, Gianluca, Moncelli, Maria Rosa
Format: Artigo
Jezik:Inglês
Izdano: 2009
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2928698/
https://ncbi.nlm.nih.gov/pubmed/19559032
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2009.06.044
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