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High-yield Heterologous Expression of Wild Type and Mutant Ca(2+) ATPase: Characterization of Ca(2+) Binding Sites by Charge Transfer
High-yield heterologous SERCA1 (Ca(2+) ATPase) expression was obtained in COS-1 cells infected with recombinant adenovirus vector (rAdSERCA). Higher transcription and expression were obtained in the presence of a His(6) tag at the amino terminus, as compared with a His(6) tag at the carboxyl SERCA t...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2928698/ https://ncbi.nlm.nih.gov/pubmed/19559032 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2009.06.044 |
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