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Directed evolution of recombinase specificity by split gene reassembly
The engineering of new enzymes that efficiently and specifically modify DNA sequences is necessary for the development of enhanced gene therapies and genetic studies. To address this need, we developed a robust strategy for evolving site-specific recombinases with novel substrate specificities. In t...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2896519/ https://ncbi.nlm.nih.gov/pubmed/20194120 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq125 |
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