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Expanding the zinc-finger recombinase repertoire: directed evolution and mutational analysis of serine recombinase specificity determinants

The serine recombinases are a diverse family of modular enzymes that promote high-fidelity DNA rearrangements between specific target sites. Replacement of their native DNA-binding domains with custom-designed Cys(2)–His(2) zinc-finger proteins results in the creation of engineered zinc-finger recom...

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Detalhes bibliográficos
Main Authors: Sirk, Shannon J., Gaj, Thomas, Jonsson, Andreas, Mercer, Andrew C., Barbas, Carlos F.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3985619/
https://ncbi.nlm.nih.gov/pubmed/24452803
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt1389
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