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Directed evolution of the site specificity of Cre recombinase
Cre recombinase from bacteriophage P1 recognizes a 34-bp recombination site, loxP, with exquisite sequence specificity and catalyzes the site-specific insertion, excision, or rearrangement of DNA. To better understand the molecular basis of protein–DNA recognition and generate recombinases with alte...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The National Academy of Sciences
2002
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC123623/ https://ncbi.nlm.nih.gov/pubmed/11904359 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.022039799 |
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