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Directed evolution of an enhanced and highly efficient FokI cleavage domain for Zinc Finger Nucleases
Zinc finger nucleases (ZFNs) are powerful tools for gene therapy and genetic engineering. The high specificity and affinity of these chimeric enzymes are based on custom-designed zinc finger proteins (ZFPs). In order to improve the performance of existing ZFN technology, we developed an in vivo evol...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2885538/ https://ncbi.nlm.nih.gov/pubmed/20447404 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2010.04.060 |
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