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Modulation of the Active Complex Assembly and Turnover Rate by Protein–DNA Interactions in Cre–LoxP Recombination,

Cre promotes recombination at the 34 bp LoxP sequence. Substitution of a critical C-G base pair in LoxP with an A-T base pair, to give LoxAT, reduced Cre binding in vitro and abolished recombination in vivo. We demonstrated that LoxAT can be recombined in vitro. However, Cre discriminates against th...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Martin, Shelley S., Chu, Victor C., Baldwin, Enoch
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2003
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2885442/
https://ncbi.nlm.nih.gov/pubmed/12779336
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi0272306
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