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Multiple Levels of Affinity-Dependent DNA Discrimination in Cre-LoxP Recombination
Cre recombinase residue Arg259 mediates a canonical bidentate hydrogen-bonded contact with Gua27 of its LoxP DNA substrate. Substituting Cyt8-Gua27 with the three other basepairs, to give LoxAT, LoxTA, and LoxGC, reduced Cre-mediated recombination in vitro, with the preference order of Gua27 >Ade...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2891539/ https://ncbi.nlm.nih.gov/pubmed/17014075 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi0605235 |
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