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Modulation of the Active Complex Assembly and Turnover Rate by Protein–DNA Interactions in Cre–LoxP Recombination,

Cre promotes recombination at the 34 bp LoxP sequence. Substitution of a critical C-G base pair in LoxP with an A-T base pair, to give LoxAT, reduced Cre binding in vitro and abolished recombination in vivo. We demonstrated that LoxAT can be recombined in vitro. However, Cre discriminates against th...

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Hlavní autoři: Martin, Shelley S., Chu, Victor C., Baldwin, Enoch
Médium: Artigo
Jazyk:Inglês
Vydáno: 2003
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2885442/
https://ncbi.nlm.nih.gov/pubmed/12779336
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi0272306
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