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Engineering Thrombin for Selective Specificity toward Protein C and PAR1
Thrombin elicits functional responses critical to blood homeostasis by interacting with diverse physiological substrates. Ala-scanning mutagenesis of 97 residues covering 53% of the solvent accessible surface area of the enzyme identifies Trp(215) as the single most important determinant of thrombin...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Biochemistry and Molecular Biology
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2885193/ https://ncbi.nlm.nih.gov/pubmed/20404340 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.119875 |
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