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DMSO and Betaine Greatly Improve Amplification of GC-Rich Constructs in De Novo Synthesis
In Synthetic Biology, de novo synthesis of GC-rich constructs poses a major challenge because of secondary structure formation and mispriming. While there are many web-based tools for codon optimizing difficult regions, no method currently exists that allows for potentially phenotypically important...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Public Library of Science
2010
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2883997/ https://ncbi.nlm.nih.gov/pubmed/20552011 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0011024 |
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