Chemical Variant of 7-deaza-dGTP for Improved GC-rich PCR and Amplification

PCR amplification of nucleic acids is a fundamental technique used in many molecular biology laboratories. Despite its widespread use, GC-rich regions of DNA sequence remain a challenge for amplification. Sequences high in GC content can form strong secondary structure, which prevents strand denatur...

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Bibliographic Details
Main Authors: Paul, Natasha, Zon, Gerry, Hogrefe, Richard, Lebedev, Alexandre, Shore, Sabrina
Format: Artigo
Language:Inglês
Published: Association of Biomolecular Resource Facilities 2013
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Poster Session Abstracts
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3635285/
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