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Chemical Variant of 7-deaza-dGTP for Improved GC-rich PCR and Amplification
PCR amplification of nucleic acids is a fundamental technique used in many molecular biology laboratories. Despite its widespread use, GC-rich regions of DNA sequence remain a challenge for amplification. Sequences high in GC content can form strong secondary structure, which prevents strand denatur...
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| Main Authors: | , , , , |
|---|---|
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Association of Biomolecular Resource Facilities
2013
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3635285/ |
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