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Betaine improves the PCR amplification of GC-rich DNA sequences.

Betaine improves the co-amplification of the two alternatively spliced variants of the prostate-specific membrane antigen mRNA as well as the amplification of the coding cDNA region of c-jun. It is suggested that betaine improves the amplification of these genes by reducing the formation of secondar...

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Autori principali: Henke, W, Herdel, K, Jung, K, Schnorr, D, Loening, S A
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1997
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC146979/
https://ncbi.nlm.nih.gov/pubmed/9380524
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