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Betaine improves the PCR amplification of GC-rich DNA sequences.

Betaine improves the co-amplification of the two alternatively spliced variants of the prostate-specific membrane antigen mRNA as well as the amplification of the coding cDNA region of c-jun. It is suggested that betaine improves the amplification of these genes by reducing the formation of secondar...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Henke, W, Herdel, K, Jung, K, Schnorr, D, Loening, S A
Format: Artigo
Language:Inglês
Published: Oxford University Press 1997
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146979/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9380524/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.19.3957
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