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Betaine, Dimethyl Sulfoxide, and 7-Deaza-dGTP, a Powerful Mixture for Amplification of GC-Rich DNA Sequences

Currently, polymerase chain reaction is the most used technique in many laboratories for either diagnostic or molecular biology purposes. Despite the large number of DNA sequences that can be easily analyzed, some GC-rich sequences are refractory to amplification due to the formation of secondary in...

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Detalhes bibliográficos
Main Authors: Musso, Marco, Bocciardi, Renata, Parodi, Sara, Ravazzolo, Roberto, Ceccherini, Isabella
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Investigative Pathology 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1876170/
https://ncbi.nlm.nih.gov/pubmed/17065422
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2006.060058
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