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Betaine, Dimethyl Sulfoxide, and 7-Deaza-dGTP, a Powerful Mixture for Amplification of GC-Rich DNA Sequences

Currently, polymerase chain reaction is the most used technique in many laboratories for either diagnostic or molecular biology purposes. Despite the large number of DNA sequences that can be easily analyzed, some GC-rich sequences are refractory to amplification due to the formation of secondary in...

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Bibliografiske detaljer
Main Authors: Musso, Marco, Bocciardi, Renata, Parodi, Sara, Ravazzolo, Roberto, Ceccherini, Isabella
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Investigative Pathology 2006
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1876170/
https://ncbi.nlm.nih.gov/pubmed/17065422
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2006.060058
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