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DMSO and Betaine Greatly Improve Amplification of GC-Rich Constructs in De Novo Synthesis
In Synthetic Biology, de novo synthesis of GC-rich constructs poses a major challenge because of secondary structure formation and mispriming. While there are many web-based tools for codon optimizing difficult regions, no method currently exists that allows for potentially phenotypically important...
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| Hlavní autoři: | , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Public Library of Science
2010
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2883997/ https://ncbi.nlm.nih.gov/pubmed/20552011 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0011024 |
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