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Dimer–monomer equilibrium of human thymidylate synthase monitored by fluorescence resonance energy transfer
An ad hoc bioconjugation/fluorescence resonance energy transfer (FRET) assay has been designed to spectroscopically monitor the quaternary state of human thymidylate synthase dimeric protein. The approach enables the chemoselective engineering of allosteric residues while preserving the native prote...
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| Glavni autori: | , , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Wiley Subscription Services, Inc., A Wiley Company
2010
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2868244/ https://ncbi.nlm.nih.gov/pubmed/20306493 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.379 |
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