Developing Multiplexed Assays for Troponin I and Interleukin-33 in Plasma by Peptide Immunoaffinity Enrichment and Targeted Mass Spectrometry

BACKGROUND: Protein biomarker candidates from discovery proteomics must be quantitatively verified in patient samples before they can progress to clinical validation. Here we demonstrate that peptide immunoaffinity enrichment coupled with stable isotope dilution mass spectrometry (SISCAPA-MRM) can b...

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Detalhes bibliográficos
Main Authors: Kuhn, Eric, Addona, Terri, Keshishian, Hasmik, Burgess, Michael, Mani, D.R., Lee, Richard T., Sabatine, Marc S., Gerszten, Robert E., Carr, Steven A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2865473/
https://ncbi.nlm.nih.gov/pubmed/19372185
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1373/clinchem.2009.123935
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