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Selecting RNA aptamers for synthetic biology: investigating magnesium dependence and predicting binding affinity

The ability to generate RNA aptamers for synthetic biology using in vitro selection depends on the informational complexity (IC) needed to specify functional structures that bind target ligands with desired affinities in physiological concentrations of magnesium. We investigate how selection for hig...

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Hlavní autoři: Carothers, James M., Goler, Jonathan A., Kapoor, Yuvraaj, Lara, Lesley, Keasling, Jay D.
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2010
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2860116/
https://ncbi.nlm.nih.gov/pubmed/20159999
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq082
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