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Selecting RNA aptamers for synthetic biology: investigating magnesium dependence and predicting binding affinity

The ability to generate RNA aptamers for synthetic biology using in vitro selection depends on the informational complexity (IC) needed to specify functional structures that bind target ligands with desired affinities in physiological concentrations of magnesium. We investigate how selection for hig...

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Bibliografske podrobnosti
Main Authors: Carothers, James M., Goler, Jonathan A., Kapoor, Yuvraaj, Lara, Lesley, Keasling, Jay D.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2010
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2860116/
https://ncbi.nlm.nih.gov/pubmed/20159999
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq082
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