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Concentration of receptor and ligand revisited in a modified receptor binding protocol for high-affinity radioligands: [(3)H]spiperone binding to D(2) and D(3) dopamine receptors

In receptor binding assays with ultra-high-affinity radioligands, it is difficult, in practice, to adhere to the golden rule that the receptor concentration in the assay should be substantially (at least 10-fold) lower than the dissociation constant (K(d)) of the radioligand and inhibition constant...

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Bibliografske podrobnosti
Main Authors: Zhen, Juan, Antonio, Tamara, Dutta, Aloke K., Reith, Maarten E.A.
Format: Artigo
Jezik:Inglês
Izdano: 2010
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2846987/
https://ncbi.nlm.nih.gov/pubmed/20122961
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jneumeth.2010.01.031
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