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Concentration of receptor and ligand revisited in a modified receptor binding protocol for high-affinity radioligands: [(3)H]spiperone binding to D(2) and D(3) dopamine receptors
In receptor binding assays with ultra-high-affinity radioligands, it is difficult, in practice, to adhere to the golden rule that the receptor concentration in the assay should be substantially (at least 10-fold) lower than the dissociation constant (K(d)) of the radioligand and inhibition constant...
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| Hlavní autoři: | , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
2010
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2846987/ https://ncbi.nlm.nih.gov/pubmed/20122961 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jneumeth.2010.01.031 |
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