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Mitotic chromosomes are constrained by topoisomerase II–sensitive DNA entanglements

We have analyzed the topological organization of chromatin inside mitotic chromosomes. We show that mitotic chromatin is heavily self-entangled through experiments in which topoisomerase (topo) II is observed to reduce mitotic chromosome elastic stiffness. Single chromosomes were relaxed by 35% by e...

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Autori principali: Kawamura, Ryo, Pope, Lisa H., Christensen, Morten O., Sun, Mingxuan, Terekhova, Ksenia, Boege, Fritz, Mielke, Christian, Andersen, Anni H., Marko, John F.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The Rockefeller University Press 2010
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2835934/
https://ncbi.nlm.nih.gov/pubmed/20194637
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.200910085
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