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Method to eliminate linear DNA from mixture containing nicked-circular, supercoiled, and linear plasmid DNA
Preparations of circular plasmid DNA in either supercoiled or nicked-circular form often are contaminated with undesired linear DNA fragments arising from shearing/degradation of chromosomal DNA or linearization of plasmid DNA itself. We report a simple enzymatic method, using a combination of λ exo...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2008
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2832929/ https://ncbi.nlm.nih.gov/pubmed/18638445 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2008.06.037 |
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