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Novel Method For Quantifying Radiation-Induced Single-Strand-Break Yields In Plasmid DNA Highlights Tenfold Discrepancy
The widely used agarose gel electrophoresis method for assessing radiation-induced single-strand-break (SSB) yield in plasmid DNA involves measurement of the fraction of relaxed-circular (C) form that migrates independently from the intact supercoiled (SC) form. We rationalized that this method may...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3184882/ https://ncbi.nlm.nih.gov/pubmed/21741945 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.06.023 |
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