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Introduction of a leucine half-zipper engenders multiple high-quality crystals of a recalcitrant tRNA synthetase

Although Escherichia coli alanyl-tRNA synthetase was among the first tRNA synthetases to be sequenced and extensively studied by functional analysis, it has proved to be recalcitrant to crystallization. This challenge remained even for crystallization of the catalytic fragment. By mutationally intro...

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Hlavní autoři: Guo, Min, Shapiro, Ryan, Schimmel, Paul, Yang, Xiang-Lei
Médium: Artigo
Jazyk:Inglês
Vydáno: International Union of Crystallography 2010
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2827346/
https://ncbi.nlm.nih.gov/pubmed/20179335
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S0907444909055462
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