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Introduction of a leucine half-zipper engenders multiple high-quality crystals of a recalcitrant tRNA synthetase

Although Escherichia coli alanyl-tRNA synthetase was among the first tRNA synthetases to be sequenced and extensively studied by functional analysis, it has proved to be recalcitrant to crystallization. This challenge remained even for crystallization of the catalytic fragment. By mutationally intro...

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Autors principals: Guo, Min, Shapiro, Ryan, Schimmel, Paul, Yang, Xiang-Lei
Format: Artigo
Idioma:Inglês
Publicat: International Union of Crystallography 2010
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2827346/
https://ncbi.nlm.nih.gov/pubmed/20179335
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S0907444909055462
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