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Targeted mutation of the Hprt gene in mouse embryonic stem cells.

The hypoxanthine-guanine phosphoribosyltransferase (Hprt) gene has been mutated in mouse blastocyst-derived embryonic stem cells by site-directed homologous recombination. Embryonic stem cells were electroporated in the presence of a targeting DNA fragment containing two specific features: (i) The t...

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Detalhes bibliográficos
Main Authors: Doetschman, T, Maeda, N, Smithies, O
Formato: Artigo
Idioma:Inglês
Publicado em: 1988
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC282503/
https://ncbi.nlm.nih.gov/pubmed/3186749
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