Real-time NMR Study of Three Small GTPases Reveals That Fluorescent 2′(3′)-O-(N-Methylanthraniloyl)-tagged Nucleotides Alter Hydrolysis and Exchange Kinetics

The Ras family of small GTPases control diverse signaling pathways through a conserved “switch” mechanism, which is turned on by binding of GTP and turned off by GTP hydrolysis to GDP. Full understanding of GTPase switch functions requires reliable, quantitative assays for nucleotide binding and hyd...

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Main Authors: Mazhab-Jafari, Mohammad T., Marshall, Christopher B., Smith, Matthew, Gasmi-Seabrook, Geneviève M. C., Stambolic, Vuk, Rottapel, Robert, Neel, Benjamin G., Ikura, Mitsuhiko
Format: Artigo
Jezik:Inglês
Izdano: American Society for Biochemistry and Molecular Biology 2010
Teme:
Reports
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2820739/
https://ncbi.nlm.nih.gov/pubmed/20018863
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.C109.064766
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