A rapid and sensitive method to detect siRNA-mediated mRNA cleavage in vivo using 5′ RACE and a molecular beacon probe

Specific detection of mRNA cleavage by 5′RACE is the only method to confirm the knockdown of mRNA by RNA interference, but is rarely reported for in vivo studies. We have combined 5′-RNA-linker-mediated RACE (5′-RLM-RACE) with real-time PCR using a molecular beacon to develop a rapid and specific me...

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Bibliografske podrobnosti
Main Authors: Lasham, Annette, Herbert, Mike, Coppieters ‘t Wallant, Natacha, Patel, Rachna, Feng, Sheryl, Eszes, Marika, Cao, Helen, Reid, Glen
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2010
Teme:
Methods Online
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2817477/
https://ncbi.nlm.nih.gov/pubmed/19942683
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp1076
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