Purification of ras GTPase activating protein from bovine brain.

In cytosolic extracts of bovine brain, we detected ras GTPase activating protein (GAP) activity that stimulated the GTP hydrolytic activity of normal c-Ha-ras p21 but not that of the oncogenic [Val12]p21 variant. GAP was purified 19,500-fold by a five-column procedure involving DEAE-Sephacel, Sephar...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Gibbs, J B, Schaber, M D, Allard, W J, Sigal, I S, Scolnick, E M
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1988
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC281680/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3293047/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.14.5026
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