Purification of ras GTPase activating protein from bovine brain.

In cytosolic extracts of bovine brain, we detected ras GTPase activating protein (GAP) activity that stimulated the GTP hydrolytic activity of normal c-Ha-ras p21 but not that of the oncogenic [Val12]p21 variant. GAP was purified 19,500-fold by a five-column procedure involving DEAE-Sephacel, Sephar...

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發表在:	Proc Natl Acad Sci U S A
Principais autores:	Gibbs, J B, Schaber, M D, Allard, W J, Sigal, I S, Scolnick, E M
格式:	Artigo
語言:	Inglês
出版:	National Academy of Sciences 1988
主題:	Research Article
在線閱讀:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC281680/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3293047/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.14.5026
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