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Purification of ras GTPase activating protein from bovine brain.

In cytosolic extracts of bovine brain, we detected ras GTPase activating protein (GAP) activity that stimulated the GTP hydrolytic activity of normal c-Ha-ras p21 but not that of the oncogenic [Val12]p21 variant. GAP was purified 19,500-fold by a five-column procedure involving DEAE-Sephacel, Sephar...

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Bibliografische gegevens
Gepubliceerd in:Proc Natl Acad Sci U S A
Hoofdauteurs: Gibbs, J B, Schaber, M D, Allard, W J, Sigal, I S, Scolnick, E M
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: National Academy of Sciences 1988
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Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC281680/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3293047/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.14.5026
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