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Targeted Mutagenesis in Zebrafish Using Customized Zinc Finger Nucleases

Zebrafish mutants have traditionally been obtained using random mutagenesis or retroviral insertions, methods that cannot be targeted to a specific gene and require laborious gene mapping and sequencing. Recently, we and others have shown that customized zinc finger nucleases (ZFNs) can introduce ta...

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Autori principali: Foley, Jonathan E., Maeder, Morgan L., Pearlberg, Joseph, Joung, J. Keith, Peterson, Randall T., Yeh, Jing-Ruey J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2009
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2814337/
https://ncbi.nlm.nih.gov/pubmed/20010934
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.209
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