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Targeted Mutagenesis in Zebrafish Using Customized Zinc Finger Nucleases
Zebrafish mutants have traditionally been obtained using random mutagenesis or retroviral insertions, methods that cannot be targeted to a specific gene and require laborious gene mapping and sequencing. Recently, we and others have shown that customized zinc finger nucleases (ZFNs) can introduce ta...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2814337/ https://ncbi.nlm.nih.gov/pubmed/20010934 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.209 |
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