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High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases
We report here an efficient method for targeted mutagenesis of Arabidopsis genes through regulated expression of zinc finger nucleases (ZFNs)—enzymes engineered to create DNA double-strand breaks at specific target loci. ZFNs recognizing the Arabidopsis ADH1 and TT4 genes were made by Oligomerized P...
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| Autores principales: | , , , , , , , , , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
2010
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2900673/ https://ncbi.nlm.nih.gov/pubmed/20508152 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0914991107 |
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