High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases

We report here an efficient method for targeted mutagenesis of Arabidopsis genes through regulated expression of zinc finger nucleases (ZFNs)—enzymes engineered to create DNA double-strand breaks at specific target loci. ZFNs recognizing the Arabidopsis ADH1 and TT4 genes were made by Oligomerized P...

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Dettagli Bibliografici
Autori principali: Zhang, Feng, Maeder, Morgan L., Unger-Wallace, Erica, Hoshaw, Justin P., Reyon, Deepak, Christian, Michelle, Li, Xiaohong, Pierick, Christopher J., Dobbs, Drena, Peterson, Thomas, Joung, J. Keith, Voytas, Daniel F.
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2010
Soggetti:
Biological Sciences
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2900673/
https://ncbi.nlm.nih.gov/pubmed/20508152
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0914991107
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