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Intended transcriptional silencing with siRNA results in gene repression through sequence-specific off-targeting

Transcriptional gene silencing has been reported with siRNA targeting the promoter region of genes. We tested several siRNAs directed against the human VEGF promoter. Of these, siVFp(−992) exhibited ≥50% suppression of VEGF production in two human cell lines. To determine the specificity of this siR...

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Autori principali: Moses, Joshua, Goodchild, Amber, Rivory, Laurent P.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Cold Spring Harbor Laboratory Press 2010
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2811671/
https://ncbi.nlm.nih.gov/pubmed/20026621
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1808510
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