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Selections that optimize RNA display in the yeast three-hybrid system

The yeast three-hybrid system (Y3H) is a powerful tool to select or confirm RNA–protein interactions. Target protein recognition of an RNA insert within a test transcript depends on at least three factors: intrinsic protein affinity for the properly folded insert, retention of RNA insert tertiary st...

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Autors principals: Wurster, Susan E., Maher, L. James
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 2010
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2811653/
https://ncbi.nlm.nih.gov/pubmed/20008486
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1880410
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