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Combining SELEX and the yeast three-hybrid system for in vivo selection and classification of RNA aptamers
Aptamers are small nucleic acid ligands that bind to their targets with specificity and high affinity. They are generated by a combinatorial technology, known as SELEX. This in vitro approach uses iterative cycles of enrichment and amplification to select binders from nucleic acid libraries of high...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1831868/ https://ncbi.nlm.nih.gov/pubmed/17283213 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.334307 |
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