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Selections that optimize RNA display in the yeast three-hybrid system
The yeast three-hybrid system (Y3H) is a powerful tool to select or confirm RNA–protein interactions. Target protein recognition of an RNA insert within a test transcript depends on at least three factors: intrinsic protein affinity for the properly folded insert, retention of RNA insert tertiary st...
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| Hauptverfasser: | , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Cold Spring Harbor Laboratory Press
2010
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2811653/ https://ncbi.nlm.nih.gov/pubmed/20008486 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1880410 |
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