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pH-Dependent Association of Proteins. The Test Case of Monoclonal Antibody HyHEL-5 and its Antigen Hen Egg White Lysozyme

We describe a method for determining diffusion-controlled rate constants for protein-protein association that explicitly includes the solution pH. The method combines the transient-complex theory for computing electrostatically enhanced association rates with an approach based on a rigorous thermody...

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Hlavní autoři: Długosz, Maciej, Antosiewicz, Jan M., Trylska, Joanna
Médium: Artigo
Jazyk:Inglês
Vydáno: 2009
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2802180/
https://ncbi.nlm.nih.gov/pubmed/19883097
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jp906829z
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