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Minimal-length short hairpin RNAs: The relationship of structure and RNAi activity
Small hairpin RNAs (shRNAs) are widely used in RNAi studies and typically consist of a stem of 19–29 base pairs (bp), a loop of at least 4 nucleotides (nt), and a dinucleotide overhang at the 3′ end. Compared with shRNAs with 21–29 bp stems, we have found that shRNAs with 19-bp or shorter stems (ssh...
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| Main Authors: | , , , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Cold Spring Harbor Laboratory Press
2010
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2802021/ https://ncbi.nlm.nih.gov/pubmed/19952116 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1894510 |
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