The Effect of Primer-Template Mismatches on the Detection and Quantification of Nucleic Acids Using the 5′ Nuclease Assay

Real-time polymerase chain reaction (PCR) is the current method of choice for detection and quantification of nucleic acids, especially for molecular diagnostics. Complementarity between primers and template is often crucial for PCR applications, as mismatches can severely reduce priming efficiency....

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Tallennettuna:
Bibliografiset tiedot
Päätekijät: Stadhouders, Ralph, Pas, Suzan D., Anber, Jeer, Voermans, Jolanda, Mes, Ted H.M., Schutten, Martin
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: American Society for Investigative Pathology 2010
Aiheet:
Regular Articles
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC2797725/
https://ncbi.nlm.nih.gov/pubmed/19948821
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2010.090035
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