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Quantification of the Detrimental Effect of a Single Primer-Template Mismatch by Real-Time PCR Using the 16S rRNA Gene as an Example

We investigated the effects of internal primer-template mismatches on the efficiency of PCR amplification using the 16S rRNA gene as the model template DNA. We observed that the presence of a single mismatch in the second half of the primer extension sequence can result in an underestimation of up t...

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Bibliografiske detaljer
Main Authors: Bru, D., Martin-Laurent, F., Philippot, L.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2008
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2258636/
https://ncbi.nlm.nih.gov/pubmed/18192413
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02403-07
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